Original Article

Year : 2023 | Volume : 11 | Issue : 2 | Page : 61-68

Comparative evaluation of anti-bacterial effect of Caesalpinia sappan aqueous extract and 0.12% Chlorhexidine against Streptococcus mutans – An in vitro study

Madhivathani S M 1 Umesh K 2 Sangeeta Chavan 3 Palanivel Pandian R 4 Prem Kumar P 5

1* Post Graduate student, Department of Public Health Dentistry, Best Dental Science College and Hospital, Madurai, Tamil Nadu, 2 Professor and Head, Department of Public Health Dentistry, RVS Dental College and Hospital, Coimbatore, Tamil Nadu, 3 Professor and Head, Department of Public Health Dentistry, Best Dental Science College and Hospital, Madurai, Tamil Nadu, 4 Reader, Department of Public Health Dentistry, Best Dental Science College and Hospital, Madurai, Tamil Nadu 5 Senior Lecturer, Department of Public Health Dentistry, Best Dental Science College and Hospital, Madurai, Tamil Nadu

Address for Correspondence:

Madhivathani S M,

Post Graduate student, Department of Public Health Dentistry, Best Dental Science College and Hospital, Madurai, Tamil Nadu.

Email-Id: madhikuzhali01@gmail.com

Abstract

Introduction :

To evaluate and compare the antimicrobial activity of various concentration of C. sappan wood aqueous extract (100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, 500mg/ml and 600mg/ml) and 0.12% chlorhexidine mouthwash using agar well diffusion method against St. mutans.

Materials and Methods :

A comparative experimental invitro study was conducted to evaluate and compare antimicrobial activity of various concentration of C. sappan aqueous extract and 0.12% Chlorhexidine. The study enclosed extract of 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, 500mg/ml, 600mg/ml and 0.12% Chlorhexidine as control. The antimicrobial activity was assessed by agar well diffusion method, were 50µl of each concentration was poured in the prepared agar well and assessed for zone of inhibition.

Results :

The antimicrobial potential of test compounds was estimated by measuring the zone of inhibition and all the procedure are done in triplicates and values are assessed using One way ANOVA and Post Hoc analysis. All the concentration used showed zone of inhibition against St. mutans. At the concentration, 600mg/ml of aqueous C. sappan extract have a larger zone of inhibition (22.41±.32). In agar well diffusion method, at the minimal concentration of 600mg/ml and C. sappan aqueous extract was comparatively better than positive control group (0.12%cholorhexidine).

Conclusion :

C. sappan wood aqueous extract could be preferred for routine oral hygiene practice where it can provide maximal antimicrobial effect without side effects of routine aid.

Keywords: Pathimugam, Antibacterial activity, C. sappan wood, Caries prevention

Financial support and sponsorship

Nil

Conflicts of interest

How to Cite this article: Madhivathani S M, K, U., Chavan, S., R, P. P., & P, P. K. (2023). Comparative evaluation of anti-bacterial effect of Caesalpinia sappan aqueous extract and 0.12% Chlorhexidine against Streptococcus mutans – An in vitro study: Original Article. International Journal of Community Dentistry, 11(2), 61–68. https://doi.org/10.56501/intjcommunitydent.v11i2.867

Original Article

Year : 2023 | Volume : 11 | Issue : 1 | Page : 50-60

Clinical estimation of the anticaries effect of probiotic toothpaste among 18-25 years old young adults- A double-blinded randomized controlled trial

Balambiga B 1 Umesh K 2 Sangeeta Chavan 3 Palanivel Pandian R 4 Prem Kumar P 5

1* Post Graduate student, Department of Public Health Dentistry, Best Dental Science College and Hospital, Madurai, Tamil Nadu, 2 Professor and Head, Department of Public Health Dentistry, RVS Dental College and Hospital, Coimbatore, Tamil Nadu, 3 Professor and Head, Department of Public Health Dentistry, Best Dental Science College and Hospital, Madurai, Tamil Nadu, 4 Reader, Department of Public Health Dentistry, Best Dental Science College and Hospital, Madurai, Tamil Nadu, 5 Senior Lecturer, Department of Public Health Dentistry, Best Dental Science College and Hospital, Madurai, Tamil Nadu

Address for Correspondence:

Balambiga B,

Post Graduate student, Department of Public Health Dentistry, Best Dental Science College and Hospital, Madurai, Tamil Nadu.

Email-Id: bala19967396@gmail.com

Abstract

Aim & Objectives:

The aim and objective of the study was to estimate the anticaries effect of probiotic toothpaste by assessing the Streptococcus mutans and Lactobacillus levels and by recording indices before and after intervention among 18-25 years old young adults.

Materials and Methods :

A double-blinded, randomized controlled trial was carried out among thirty healthy volunteers of young adults in the city of Madurai. The subjects were randomly divided into two equal groups of A and B. At baseline, samples of saliva were collected to check Streptococcus mutans and Lactobacillus levels and indices like Oral Hygiene Index (1960), Modified Turesky Plaque Index (1970), Stain Index (1968) and Gingival Index (1963) were recoded. Group A received Conventional toothpastes (Colgate cavity protection) and Group B received Probiotic toothpaste (Purexa). For the next 15 days, they were instructed to brush using only the allotted toothpastes. The saliva sample collection and recording of indices were repeated after 15 days post-intervention.

Results :

Gingival and oral hygiene indices showed significant differences of mean in Group B (0.14, 0.28; p<0.05 respectively). A significant difference of means was noted between Group A and B in the case of Plaque, Stain, Oral Hygiene indices and the bacterial count (Lactobacilli and Streptococcus mutans) (A :0.10, -0.12, 0.01; B :0.06, 0.09, 0.28 and -12.8, -14.8 ; p<0.05 respectively).

Conclusion :

The probiotic toothpastes illustrated a significant reduction of Lactobacillus, Streptococcus mutans bacterial count, Stain, Plaque and Oral Hygiene index scores compared to conventional toothpastes.

Keywords: probiotic, anticaries, toothpaste, adults

Financial support and sponsorship

Nil

Conflicts of interest

There are no conflicts of interest

How to Cite this article : B, B., Umesh K, Sangeeta Chavan, R Palanivel Pandian, & P Prem Kumar. (2023). Clinical estimation of anticaries effect of probiotic toothpaste among 18-25 years old young adults- A double blinded randomized controlled trial. International Journal of Community Dentistry, 11(1), 50–60. https://doi.org/10.56501/intjcommunitydent.v11i1.852