Original Research
Keywords: Class II malocclusion, genetics, epigenetics, gene expression, regulators, microRNAs.
Year : 2023 | Volume : 14 | Issue : 3 | Page : 40-55
MicroRNA regulators of candidate genes involved in Class II skeletal malocclusion - A data mining approach.
Ashwin Mathew George 1, Anitha P2, Sumathi Felicita A3, Vijayashree Priyadarshini J4, Prasanna Arvind T.R.5 2, 4 Professor, Clinical Genetics Lab, Saveetha Dental College, SIMATS, Saveetha University. 1,3 Professor, 5 Senior Lecturer, Department of Orthodontics, Saveetha Dental College, SIMATS, Saveetha University.
Address for Correspondence:
Jayaseelan Vijayashree Priyadarshini,
Professor, Clinical Genetics Lab, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, SIMATS University
Email id: vijayashreej.sdc@saveetha.com
Abstract
Background:
Epigenetic regulators play a vital role in determining a complex phenotype. The Skeletal Class II malocclusion is one such phenotype, which is a polygenic, complex disorder. The identification of epigenetic regulators would aid in understanding the complex relationship between the epigenetic marks and the phenotype. Also, these epigenetic marks can be considered for developing diagnostic leads upon validation for a specific disorder.
Materials and methods:
The present study follows an observational study design, which was performed using computational tools. The preliminary data about the genes associated with the Skeletal class II malocclusion was derived from DisGeNet, followed by the identification of the protein-protein interaction networks. The microRNA targets were then identified using miRDB and the unique microRNA population common to all five genes was further curated using the Venn plot.
Results:
The DisGeNet database provided information on the genes that were associated with skeletal Class II malocclusion. The five genes identified were ACTN3, GH1, HDAC4, HMGA2 and KAT6B. One microRNA, hsa-miR-892c-5p was unique to ACTN3, HDAC4, and HMGA2. The hsa-miR-3925-5p and hsa-miR-590-3p were found to be common to the genes ACTN3, HDAC4, and GH1 + HMGA2 respectively.
Discussion:
The identification of microRNAs targeting candidate genes could aid in defining the role of these microRNAs in establishing the phenotype. The future scope of this study lies in curating microRNAs that are common to class II malocclusion-related candidate genes. This panel of differentially expressed microRNAs can further be developed as an early diagnostic marker, for identifying the skeletal abnormality that they would be possibly associated with.
Conflict of interest
The authors have no conflict of interest to declare.
Funding
No funding was received for the study.
How to Cite this article: George, A. M., Anitha P, A, S. F., Vijayashree Priyadarshini J, & Prasanna Arvind T.R. (2023). MicroRNA regulators of candidate genes involved in Class II skeletal malocclusion - A data mining approach.: Original Research. International Journal of Orthodontic Rehabilitation, 14(3), 40–55. https://doi.org/10.56501/intjorthodrehabil.v14i3.904
Review Article
Keywords:
Genetics, ACTN3 gene, Polymorphism, Masseter muscle, Facial height, Malocclusion
Year : 2023 | Volume : 14 | Issue : 1 | Page : 36-43
ACTN3 gene variations in various Ethnic populations and its association with Skeletal Malocclusion – A Literature Review
Ashwin Mathew George1, Nagachandran K.S.2 , Sumathi Felicita.A3 , Vijayashree Priyadarshini. J4 , Anitha. P5
1,2,3 Professor, Department of Orthodontics, Saveetha Dental College and Hospital, 4 Associate Professor, 5 Research Scholar, Clinical Genetics Lab, Centre for Cellular and Molecular research, Saveetha Dental College and Hospital
Address for Correspondence:
Nagachandran K.S., MDS
Professor, Department of Orthodontics, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, TamilNadu, India
Email: orthodrnaga21@gmail.com Phone: +91-9884152660
Abstract:
Genetic etiology of Skeletal Malocclusion has been explored extensively over recent years with the advent of advances in molecular technologies. Studies have reported that the masticatory muscles regulate skeletal development and few recent works of literature report that the muscle growth regulators influence the facial proportions in sagittal and vertical dimensions in Skeletal Class II malocclusion. This review is a humble attempt to highlight the role of one such muscle gene known as ACTN3 and its association with Skeletal Class II malocclusion.
Keywords: Genetics, ACTN3 gene, polymorphism, masseter muscle, facial height, malocclusion
Source of Funding:
Nil.
Conflict of Interest:
The authors have no conflict of interests to declare
How to cite this article: Ashwin Mathew George, Nagachandran.K.S, Sumathi Felicita.A, Vijayashree Priyadarshini.J, & Anitha.P. (2023). ACTN3 gene variations in various Ethnic populations and its association with Malocclusion – A Literature Review: Review Article. International Journal of Orthodontic Rehabilitation, 14(1), 36–43. https://doi.org/10.56501/intjorthodrehabil.v14i1.718